Two solutions are prepared:
Solution A 0.1 g of Lucigenin (commercially available e.g. from Aldrich)
in 50 ml of ethanol and 50 ml of water.
Solution B: 8 g of NaOH in 70 ml of water
and 5 ml of 1.5% H2O2-Solution
(Attention: Solution B is stable only for a short period)
In a dark room, the freshly prepared solution A is added to solution B.
A bright yellow-green light can be observed, which changes later its color to light blue.
Addition of sodium hydroxide solution and H2O2 refreshes the light for a